Question 1

During replication,each parent DNA strand serves as a ________ for synthesis of new DNA strands.

Accepted Answer

A)  copy point
B)  template
C)  comparison molecule
D)  scaffold
E)  reservoirF) C) and D)
G) A) and B)

Question 2

Split genes ________.

Accepted Answer

A)  are common in bacteria and eukaryotes
B)  only have exons initially transcribed to mRNA
C)  have introns located only at the beginning and end of a coding region
D)  use spliceosomes to excise introns and then join exons
E)  All of the choices are correct.F) None of the above
G) A) and C)

Question 3

When bacteriophages invade bacterial cells,they leave behind DNA that is recognized by the bacteria as foreign and can be eliminated in the event of another attack,reminiscent of adaptive immunity.This natural process can be adapted in the lab so that a combination of guide DNA and nucleases facilitate the cutting of DNA at a precise location in any organism.  Here we are describing ________.

Accepted Answer

A)  CRISPR
B)  gene therapy
C)  recombinant DNA technology
D)  synthetic biology
E)  metagenomicsF) A) and C)
G) A) and E)

Question 4

Viruses are often used as cloning hosts in recombinant DNA methods.

Accepted Answer

A) True 
 B)False

Question 5

DNA photolyase,in the presence of visible light,can repair DNA damage due to ultraviolet radiation.

Accepted Answer

A) True 
 B)False  True

Question 6

The expression of genetic traits is the ________.

Accepted Answer

A)  genome
B)  genotype
C)  proteome
D)  phenotype
E)  proteotypeF) B) and D)
G) A) and B)

Question 7

The various techniques by which scientists manipulate DNA in the lab are called ________.

Accepted Answer

A)  genetic engineering
B)  biotechnology
C)  recombinant DNA
D)  gel electrophoresis
E)  gene probesF) A) and D)
G) All of the above

Question 8

Eukaryotic chromosomes differ from bacterial chromosomes because only eukaryotes have ________.

Accepted Answer

A)  histone proteins
B)  chromosomes in a nucleus
C)  several to many chromosomes
D)  elongated,not circular,chromosomes
E)  All of the choices are correctF) A) and D)
G) B) and C) E

Question 9

Which of the following is not true of conjugation?

Accepted Answer

A)  It involves direct contact between cells.
B)  It transfers genes for drug resistance.
C)  It transfers genes for resistance to heavy metals.
D)  It transfers genes for a polysaccharide capsule.
E)  The donor retains a copy of the transferred genes.F) A) and B)
G) A) and C)

Question 10

Vectors often contain a gene conferring drug resistance to their cloning host,in order to detect cells harboring the plasmid.

Accepted Answer

A) True 
 B)False

Question 11

The transfer of DNA fragments from a dead cell to a live,competent recipient cell that results in a changed recipient cell is ________.

Accepted Answer

A)  transduction
B)  conjugation
C)  transformation
D)  transmission
E)  mitosisF) None of the above
G) C) and D)

Question 12

The primers in PCR are ________.

Accepted Answer

A)  synthetic short strands of DNA (oligonucleotides) 
B)  bacterial enzymes
C)  short RNA strands
D)  DNA polymerases
E)  reverse transcriptasesF) C) and D)
G) A) and B)

Question 13

Replication of DNA begins at a(n) ________ rich area.

Accepted Answer

A)  guanine-cytosine.
B)  uracil-adenine.
C)  adenine-thymine.
D)  adenine-cytosine.
E)  guanine-adenine.F) C) and D)
G) A) and D)

Question 14

Base pairs in DNA are held together by ________ bonds.

Accepted Answer

A)  peptide
B)  nonpolar covalent
C)  polar covalent
D)  hydrogen
E)  sulfhydrylF) C) and D)
G) B) and C)

Question 15

Okazaki fragments are attached to the growing end of the lagging strand by ________.

Accepted Answer

A)  DNA ligases
B)  DNA polymerases
C)  DNA helicases
D)  DNA gyrases
E)  primasesF) A) and D)
G) A) and C)

Question 16

Which of the following is not associated with a bacterial ribosome?

Accepted Answer

A)  Is a 70S
B)  Has a peptide (P) site
C)  Has an exit site
D)  Small unit provides the enzymes for making peptide bonds
E)  Has an amino acid (
A)  siteF) A) and B)
G) A) and C)

Question 17

Which is incorrect about purines?

Accepted Answer

A)  Only found in DNA,not in RNA
B)  Are nitrogenous bases
C)  Always paired with a specific pyrimidine
D)  Include adenine and guanine
E)  Found within nucleotidesF) D) and E)
G) C) and E)

Question 18

A recombinant organism is one that ________.

Accepted Answer

A)  has obtained genetic material from another organism that has integrated into the genome,combining the two DNA molecules
B)  induces a gene that was previously turned off,thereby producing a new phenotype
C)  obtains genes by conjugation,but not transduction
D)  has sustained a mutation in the genetic material such that a new phenotype results,for example,the organism develops increased virulence or antibiotic resistanceE) C) and D)
F) B) and D)

Question 19

A 59-year-old female has been diagnosed with Escherichia coli infection following the consumption of contaminated meat. The patient is exhibiting a high fever, severe abdominal cramping, and diarrhea. The RN provides supportive care and fluid resuscitation as ordered. The nurse and medical team  provide education to the patient and her family regarding the genetics of the E. coli bacterium.
-In gram-negative bacteria,like E.coli,the fertility factor is a plasmid possessed by the bacterium that will donate its DNA to a recipient cell.Which of the following statements by the patient demonstrates an understanding of this plasmid?

Accepted Answer

A)  It is a large,double-stranded linear piece of DNA.
B)  It is found in F- cells only.
C)  It controls the process of transduction in bacterial cells.
D)  It encodes the information to create a pilus.E) B) and D)
F) B) and C) D

Question 20

Which is incorrect about inducible operons?

Accepted Answer

A)  Have genes turned off by a buildup of end product
B)  Are often for catabolic pathways
C)  Are normally turned off
D)  Are turned on by the substrate of the enzyme
E)  Include the lac operonF) C) and D)
G) A) and B)

Exam 8: Microbial Genetics and Genetic Engineering

During replication,each parent DNA strand serves as a ________ for synthesis of new DNA strands.

Correct Answer
verified

Split genes ________.

Correct Answer
verified

Correct Answer
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Viruses are often used as cloning hosts in recombinant DNA methods.

Correct Answer
verified

DNA photolyase,in the presence of visible light,can repair DNA damage due to ultraviolet radiation.

Correct Answer
verified
True

The expression of genetic traits is the ________.

Correct Answer
verified

The various techniques by which scientists manipulate DNA in the lab are called ________.

Correct Answer
verified

Eukaryotic chromosomes differ from bacterial chromosomes because only eukaryotes have ________.

Correct Answer
verified
E

Which of the following is not true of conjugation?

Correct Answer
verified

Vectors often contain a gene conferring drug resistance to their cloning host,in order to detect cells harboring the plasmid.

Correct Answer
verified

The transfer of DNA fragments from a dead cell to a live,competent recipient cell that results in a changed recipient cell is ________.

Correct Answer
verified

The primers in PCR are ________.

Correct Answer
verified

Replication of DNA begins at a(n) ________ rich area.

Correct Answer
verified

Base pairs in DNA are held together by ________ bonds.

Correct Answer
verified

Okazaki fragments are attached to the growing end of the lagging strand by ________.

Correct Answer
verified

Which of the following is not associated with a bacterial ribosome?

Correct Answer
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Which is incorrect about purines?

Correct Answer
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A recombinant organism is one that ________.

Correct Answer
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Correct Answer
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D

Which is incorrect about inducible operons?

Correct Answer
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Exam 8: Microbial Genetics and Genetic Engineering

During replication,each parent DNA strand serves as a ________ for synthesis of new DNA strands.

Correct AnswerverifiedShow Answer

Split genes ________.

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Viruses are often used as cloning hosts in recombinant DNA methods.

Correct AnswerverifiedShow Answer

DNA photolyase,in the presence of visible light,can repair DNA damage due to ultraviolet radiation.

Correct AnswerverifiedTrue

The expression of genetic traits is the ________.

Correct AnswerverifiedShow Answer

The various techniques by which scientists manipulate DNA in the lab are called ________.

Correct AnswerverifiedShow Answer

Eukaryotic chromosomes differ from bacterial chromosomes because only eukaryotes have ________.

Correct AnswerverifiedE

Which of the following is not true of conjugation?

Correct AnswerverifiedShow Answer

Vectors often contain a gene conferring drug resistance to their cloning host,in order to detect cells harboring the plasmid.

Correct AnswerverifiedShow Answer

The transfer of DNA fragments from a dead cell to a live,competent recipient cell that results in a changed recipient cell is ________.

Correct AnswerverifiedShow Answer

The primers in PCR are ________.

Correct AnswerverifiedShow Answer

Replication of DNA begins at a(n) ________ rich area.

Correct AnswerverifiedShow Answer

Base pairs in DNA are held together by ________ bonds.

Correct AnswerverifiedShow Answer

Okazaki fragments are attached to the growing end of the lagging strand by ________.

Correct AnswerverifiedShow Answer

Which of the following is not associated with a bacterial ribosome?

Correct AnswerverifiedShow Answer

Which is incorrect about purines?

Correct AnswerverifiedShow Answer

A recombinant organism is one that ________.

Correct AnswerverifiedShow Answer

Correct AnswerverifiedD

Which is incorrect about inducible operons?

Correct AnswerverifiedShow Answer

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True

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E

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D

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